Braz Oral Res 22(1):5–10Īghaloo T, Cowan CM, Chou YF, Zhang X, Lee H, Miao S, Hong N, Kuroda S, Wu B, Ting K, Soo C (2006) Nell-1-induced bone regeneration in calvarial defects. Plast Reconstr Surg 118(4):862–873Īccorsi-Mendonca T, Conz MB, Barros TC, de Sena LA, Soares Gde A, Granjeiro JM (2008) Physicochemical characterization of two deproteinized bovine xenografts. In the rat calvarial bone defect experiment, histological observation showed more mature bone formed in the DBM putty group than that in the type I collagen group at 12 weeks, which indicated the bone putty prepared by DBM gel exhibited a better bone repair capability.Īcarturk TO, Hollinger JO (2006) Commercially available demineralized bone matrix compositions to regenerate calvarial critical-sized bone defects. The putty prepared by DBM gel was easy to handle without loss of DBM particles at room temperature. And the ALP production of MC3T3-E1 cells in the DBM gels group increased with prolonged culturing, the mineralized nodules formed in MC3T3-E1 cells on 14th day after co-culture. The result showed there was 2.953 ± 0.054 ng BMP contained in per gram of the DBM gel. Then the DBM putty was prepared and filled into the rat calvarial defect model to evaluate the bone repairing ability by micro-CT and histology. After the bovine DBM gel was prepared, the BMPs content as well as the ability to promote osteogenic differentiation of MC3T3-E1 cells in vitro were investigated. In order to prepare a kind of DBM putty with a good biocompatibility and bioactivity, the DBM gel was processed from the DBM and the feasibility as a carrier for the DBM putty was evaluated. Recent studies indicated the DBM putties with the various carriers were different in bone repairing ability. The demineralized bone matrix (DBM) putty is a traditional bone graft utilized to facilitate the repair and reconstruction of bone.

Matrix hair putty